Richard Wong Laboratory 金沢大学

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研究業績

最近の研究成果 (IF> 9)

We study nano-pores (nuclear pore complex (NPC)) which implanted on the nuclear envelope (NE) as the headquarters' (nucleus) gatekeepers in our cells.

  • 2024-04 We published an invited paper in Cell Host Microbe   
  • 2023-01 We published a paper in Nano Letters
  • 2022-11 We published a paper in JEV
  • 2021-12 We published a paper in JEV
  • 2021-04 We published a paper in Autophagy
  • 2020-08 We published a paper in Nano Letters
  • 2020-06 We published a paper in Biomaterials

In Kanazawa Univ., our lab members also published the NPC studies in Mol. Cancer, ACS Nano, PNAS, Cell Death Dis., Cell Rep., Cell Chem Biol., EMBO Rep., Oncogene and etc.

論文

2024

  • Sajidah ES, Lim K, Hazawa M, Wong RW*.
    Nanoimaging of SARS-CoV-2 viral invasion toward the nucleus and genome.
    Cell Rep. Phys. Sci.       2024, 5, 102111,doi: 10.1016/j.xcrp.2024.102111 .
  • Ikliptikawati DK*, Makiyama K, Hazawa M*, Wong RW*.
    Unlocking the Gateway: The Spatio-temporal Dynamic of p53 Family by the Nuclear Pores and Its Implication to the Therapeutic Approach in Cancer.
    Int. J. Mol. Sci.       2024 Jul 7;25(13):7465. doi: 10.3390/ijms25137465.
  • Matsumoto K#, Ikliptikawati DK#, Makiyama K, Mochizuki K, Tobita M, Kobayashi I, Voon DC, Lim K, Ogawa K, Kashiwakura I, Suzuki HI, Yoshino H, Wong RW, Hazawa M*.
    Phase-separated super-enhancers confer an innate radioresistance on genomic DNA.
    J Radiat Res.       2024 Jun 14:rrae044.
    doi: 10.1093/jrr/rrae044  
    # equal contribution
  • Lim K, Hazawa M, Wong RW*
    Crafty mimicry grants nuclear pore entry to HIV.
    Cell Host Microbe       2024 32 (4):441-442.
    doi: 10.1016/j.chom.2024.03.004  
  • Hazawa M*#, Ikliptikawati DK#, Iwashima Y#, Lin D-C#, Jiang Y#, Qiu Y, Makiyama K, Matsumoto K, Kobayashi A, Nishide G, Lim K, Yoshino H, Minamoto T, Suzuki T, Kobayashi I, Meguro-Horike M, Jiang Y-Y, Nishiuchi T, Konno H, Koeffler H.P., Hosomichi K, Tajima A, Horike S, Wong RW*
    Super enhancer trapping by the nuclear pore via intrinsically disordered regions of proteins in squamous cell carcinoma cells
    Cell Chemical Biology 2024 April 18; 31, 792–804. [Cover]
    doi: 10.1016/j.chembiol.2023.10.005   
    # equal contribution                                   

  • Qiu Y#, Sajidah ES#, Kondo S#, Narimatsu S, Sandira SI, Higashiguchi O, Nishide G, Taoka A, Hazawa M, Inaba Y, Inoue H, Matsushima A, Okada Y, Nakada M, Ando T, Lim K*, Wong RW*
    An efficient method for isolating and purifying nuclei from mice brain for single-molecule imaging using high-speed atomic force microscopy
    Cells   2024, 13, 279.
    doi: 10.3390/cells13030279  
    # equal contribution

  • Kato Y, Uto T, Ishizaki T, Tanaka D, Ishibashi K, Matsuda Y, Onoda I, Kobayashi A, Hazawa M, Wong RW, Takahashi K, Hirata E, Kuroda K.
    Optimization of Zwitterionic Polymers for Cell Cryopreservation.
    Macromol Biosci. 2024 Feb 8:e2300499.
    doi: 10.1002/mabi.202300499. 

2023

  • Dunajova Z, Mateu BP, Radler P#, Lim K#, Brandis D, Velicky P, Danzl JG, Wong RW, Elgeti J, Hannezo E, Loose M
    Chiral and nematic phases of flexible active filaments
    Nature Physics 2023;19(12):1916-1926.
    doi: 10.1038/s41567-023-02218-w  
  •  Kato Y, Matsuda Y, Uto T, Tanaka D, Ishibashi K, Ishizaki T, Ohta A, Kobayashi A, Hazawa M, Wong RW, Ninomiya K, Takahashi K, Hirata E, Kuroda K.
    Cell-compatible isotonic freezing media enabled by thermo-responsive osmolyte-adsorption/exclusion polymer matrices.
    Commun Chem. 2023 Nov 29;6(1):260.
    doi: 10.1038/s42004-023-01061-7.
  • Nishide G#, Lim K #, Tamura M#, Kobayashi A , Zhao Q, Hazawa M , Ando T, Nishida N*, Wong RW *
    Nanoscopic Elucidation of Spontaneous Self-Assembly of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open Reading Frame 6 (ORF6) Protein.
    J. Phys. Chem. Lett. 2023, 14, 38, 8385–8396
    doi:10.1021/acs.jpclett.3c01440
  • Ikliptikawati DK #, Hirai N#, Makiyama K#, Sabit H, Kinoshita M, Matsumoto K, Lim K, Meguro-Horike M, Horike S, Hazawa M *, Nakada M*,Wong RW *
    Nuclear transport surveillance of p53 by nuclear pores in glioblastoma.
    Cell Reports 2023 Aug 29;42(8):112882.
    doi:10.1016/j.celrep.2023.112882
  • Lim K*, Nishide G, Sajidah ES, Yamano T, Qiu　Y, Yoshida T, Kobayashi A, Hazawa M, Ando T, Hanayama R*, Wong RW*
    Nanoscopic assessment of anti-SARS-CoV-2 spike neutralizing antibody using high-speed AFM.
    Nano Letters 2023 Jan 25;23(2):619-628.
    doi: 10.1021/acs.nanolett.2c04270

2022

  • Sajidah ES (D3), Lim K *, Yamano T, Nishide G ,Qiu Y , Yoshida T,Wang H, Kobayashi A, Hazawa M, Dewi F ,Hanayama R, Ando T, Wong RW *
    Spatiotemporal tracking of small extracellular vesicle nanotopology in response to physicochemical stresses revealed by HS-AFM
    J Extracell Vesicles 2022;11:12275
    DOI: 10.1002/jev2.12275 [link]
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  • Kimura K, Yamamori S, Hazawa M, Kobayashi-Sun J, Kondo M, Wong RW , Kobayashi I.
    Inhibition of canonical Wnt signaling promotes ex vivo maintenance and proliferation of hematopoietic stem cells in zebrafish. 
    Stem Cells. 2022 Jun 27:sxac044.
    doi: 10.1093/stmcls/sxac044.
    PMID: 35759948
  • Kondo H(M2) , Mishiro K, Iwashima Y , Qiu Y , Kobayashi A , Lim K , Domoto T, Minamoto T, Ogawa K, Kunishima M, Hazawa M *, Wong RW *
    Discovery of a novel aminocyclopropenone compound that inhibits BRD4-driven nucleoporin NUP210 expression and attenuates colorectal cancer growth
    Cells (2022)
    doi: 10.3390/cells11030317
  • Pramudya M, Dewi FRP, Wong RW , Anggraini DW, Winarni D, Wahyuningsih SPA. Anti-cancer activity of an ethanolic extract of red okra pods (Abelmoschus esculentus L. Moench) in rats induced by N-methyl-N-nitrosourea
    Vet World. 2022 May;15(5):1177-1184.
    doi: 10.14202/vetworld.2022.1177-1184. PMID: 35765486
  • Makiyama K(B4 undergraduate student), Hazawa M *, Kobayashi A , Lim K , Voon VC,Wong RW*
    NSP9 of SARS-CoV-2 attenuates nuclear transport by hampering nucleoporin 62 dynamics and functions in host cells
    Biochem. Biophys. Res. Commun. 586: 137-142 [link]

2021

  • Lim K *, Nishide G(M2) , Yoshida T, Watanabe-Nakayama T,Kobayashi A ,Hazawa M, Hanayama R, Ando T, Wong RW*
    Millisecond dynamic of SARS-CoV-2 spike and its interaction with ACE2 receptor and small extracellular vesicles
    J Extracell Vesicles 2021 Dec;10(14):e12170.
    doi: 10.1002/jev2.12170. [link]
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  • Kato Y, Uto T, Tanaka D, Ishibashi K, Kobayashi A, Hazawa M , Wong RW, Ninomiya K, Takahashi K, Hirata E*, Kuroda K*.
    Synthetic zwitterions as efficient non-permeable cryoprotectants
    Comm. Chem (2021) 4:151
    doi.org/10.1038/s42004-021-00588-x [link]
  • Wong RW*
    New activities of the nuclear pore complexes. (Editorial)
    Cells 2021 Aug 18;10(8):2123.
    doi: 10.3390/cells10082123.
    PMID: 34440892 [link]
  • Hazawa M# *, Amemori S#, Nishiyama Y#, Iga Y , Iwashima Y , Kobayashi A , Nagatani H, Mizuno M, Takahashi K,Wong RW *
    A light-switching pyrene probe to detect biomolecular phase separation
    iScience 2021 Jul 15;24(8):102865.
    doi: 10.1016/j.isci.2021.102865. PMID: 34386728
    Featured on 北國新聞
    [Patent] 特願2021-093100 [link]
  • Ikliptikawati DK(D3 )#, Hazawa M #, So FT, Terada D, Kobayashi A , Segawa TF*, Shirakawa M*,Wong RW *
    Label-free tomographic imaging of nanodiamonds in living cells
    Diamond & Related Materials 2021 118:108517;
    doi.org/10.1016/j.diamond.2021.108517 [Cover] [link]
  • Sajidah ES(D1 student), Lim K * and Wong RW *
    How SARS-CoV-2 and other viruses build an invasion route to hijack the host nucleocytoplasmic trafficking system
    Cells 2021, 10(6), 1424;
    doi.org/10.3390/cells10061424s [link]
  • Nishide G , Lim K *, Mohamed M , Kobayashi A , Hazawa M , Watanabe-Nakayama T, Kodera N, Ando T and Wong RW *
    High-Speed Atomic Force Microscopy reveals spatiotemporal dynamics of histone Protein H2A involution by DNA inchworming
    J. Phys. Chem. Lett. 2021 Apr 14: 3837–3846
    doi: 10.1021/acs.jpclett.1c00697 [link]
    PMID: 33852305
    Featured on 北國新聞 科学新聞 Nature index
  • Dewi FRP , Jiapaer S, Kobayashi A , Hazawa M , I kliptikawati DK , Hartono , Sabit H, Nakada M*, Wong RW*
    Nucleoporin TPR (translocated promoter region, nuclear basket protein) upregulation alters MTOR-HSF1 trails and suppresses autophagy induction in ependymoma
    Autophagy 2021 Apr;17(4):1001-1012.
    doi: 10.1080/15548627.2020.1741318.
    PMID: 32207633 [link]
    Featured on 北國新聞
  • Fukasawa K, Kadota T, Horie T, Tokumura K, Terada R, Kitaguchi Y, Park G, Ochiai S, Iwahashi S, Okayama Y, Hiraiwa M, Yamada T, Iezaki T, Kaneda K, Yamamoto M, Kitao T, Shirahase H, Hazawa M , Wong RW , Todo T, Hirao A, Hinoi E.*
    CDK8 maintains stemness and tumorigenicity of glioma stem cells by regulating the c-MYC pathway
    Oncogene Apr;40(15):2803-2815.
    doi: 10.1038/s41388-021-01745-1.
    PMID: 33727660 [link]
  • Klionsky DJ*, Abdel-Aziz AK, Abdelfatah S, ... Wong RW et al.
    Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition),
    Autophagy 2021 Jan;17(1):1-382.
    doi: 10.1080/15548627.2020.1797280.
    PMID: 33634751 [link]
  • Kato K (B4 undergraduate student), Ikliptikawati DK , Kobayashi A *, Kondo H, Lim K , Hazawa M and Wong RW *
    Overexpression of SARS-CoV-2 protein ORF6 dislocates RAE1 and NUP98 from the nuclear pore complex
    Biochem. Biophys. Res. Commun. 2021 536:59-66.
    doi: 10.1016/j.bbrc.2020.11.115.
    PMID: 33360543 [link]

2020

  • Lim K, Kodera N, Wang H, Mohamed M ,Hazawa M ,Kobayashi A, Yoshida T, Hanayama R Yano S, Ando T,Wong RW *
    High-speed AFM reveals molecular dynamic of human influenza A hemagglutinin and its interaction with exosomes.
    Nano Letters 2020 20, 9, 6320–6328.
    doi: 10.1021/acs.nanolett.0c01755.
    PMID: 32787163 [link]
    Featured on  NHK, 北國新聞 Nature index
  • Bolidong D, Domoto T, Uehara M, Sabit H, Okumura T, Endo Y, Nakada M, Ninomiya I, Miyashita T, Wong RW , Minamoto T*
    Potential therapeutic effect of targeting glycogen synthase kinase 3β in esophageal squamous cell carcinoma
    Sci. Rep. 2020 Jul 16;10(1):11807.
    doi: 10.1038/s41598-020-68713-9.
    PMID: 32678196 [link]
  • Mohamed MS , Hazawa M , Kobayashi A , Guillaud L, Watanabe-Nakayama T, Nakayama M, Wang H , Kodera N, Oshima M, Ando T, Wong RW *
    Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core
    Biomaterials 2020 Oct; 256:120198.
    doi: 10.1016/j.biomaterials.2020.120198.
    PMID: 32622019 [link]
  • Hazawa M , Yoshino H*, Nakagawa Y, Shimizume R, Nitta K, Sato Y, Sato M, Wong RW , Kashiwakura I
    Karyopherin β1 regulates radioresistance and radiation-increased programmed death-ligand 1 expression in human head and neck squamous cell carcinoma
    Cancers     (Basel). 2020 Apr 8;12(4):908.
    doi: 10.3390/cancers12040908.
    PMID: 32276424 [link]
  • Lim K , Yong ZWE, Wang H, Tan TZ, Huang RYJ, Yamamoto D, Inaki N, Hazawa M , Wong RW , Oshima H, Oshima M, Ito Y, Voon DC *
    Inflammatory and mitogenic signals drive IL23A secretion independent of IL12B in intestinal epithelial cells
    J. Biol. Chem. 2020 May 8;295(19):6387-6400.
    doi: 10.1074/jbc.RA120.012943.
    PMID: 32209656 [link]
  • Hazawa M *, Sakai K , Kobayashi A , Yoshino H, Iga Y , Iwashima Y , Lim K , Voon DC, Jiang YY, Horike S, Lin DC, Wong RW *
    Disease-specific alteration of karyopherin-α subtype establishes　feed-forward oncogenic signaling in head and neck squamous cell carcinoma
    Oncogene 2020 Mar;39(10):2212-2223. doi: 10.1038/s41388-019-1137-3. PMID: 31822798 [link]
  • Lim K , Mohamed MS , Wang H , Hartono , Hazawa M , Kobayashi A , Voon DC, Kodera N, Ando T and Wong RW *
    Direct Visualization of Avian Influenza H5N1 Hemagglutinin Precursor and its Conformational Change by High-Speed Atomic Force Microscopy.
    Biochim Biophys Acta Gen Subj. 2020 Feb 27. pii: S0304-4165(19)30049-2.
    doi: 10.1016/j.bbagen.2019.02.015.
    PMID: 30825615 [link]

2019

  • Hartono, Hazawa M, Lim K ,Dewi FRP, Kobayashi A , and Wong RW *
    Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis.
    Cell Division 2019 Aug 3;14:7.
    doi: 10.1186/s13008-019-0050-z.
    PMID: 31388347 [link]

2018

  • Lim S and Wong RW *
    Targeting nucleoporin POM121-importin β axis in prostate cancer.
    Cell Chem. Biol. 2018 Sep 20;25(9):1056-1058.
    doi: 10.1016/j.chembiol.2018.09.003.
    PMID: 30241601 [link]
  • Dewi FRP , Domoto T, Hazawa M , Kobayashi A , Douwaki T, Minamoto T and Wong RW *
    Colorectal cancer cells require glycogen synthase kinase-3β for sustaining mitosis via translocated promoter region (TPR)-dynein interaction　 Oncotarget 2018 Jan 30;9(17):13337-13352.
    doi: 10.18632/oncotarget.24344.
    PMID: 29568361 [link]
  • Hazawa M *, Lin D, Kobayashi A , Jiang YY, Dewi FRP , Mohamed MS , Hartono , Nakada S, Meguro-Horike M, Horike S, Koeffler H and Wong RW *
    Rock-dependent phosphorylation of NUP62 regulates p63 nuclear transport and cell fate of squamous cell carcinoma.
    EMBO Rep. 2018 Jan 8; 19, 73-88.
    doi: 10.15252/embr.201744523
    PMID: 29217659 [link]
    Editorial by (News & Views): EMBO Rep .
    Featured on  北國新聞

2017

  • Mohamed MS , Kobayashi A , Taoka A, Watanabe-Nakayama T, Kikuchi Y, Hazawa M , Minamoto T, Fukumori Y, Kodera N, Uchihashi T, Ando T, and Wong RW *
    High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells.
    ACS Nano. 2017 11(6):5567-5578.
    doi: 10.1021/acsnano.7b00906.
    PMID: 28530826  [link]
    Featured on  北國新聞
  • Hazawa M *, Lin D, Handral H, Xu L, Chen Y, Jiang YY, Thippeswamy A, Ding LW, Meng X,  Sharma A, Samuel S, Movahednia M, Wong RW , Yang H, Tong C, and Koeffler H
    ZNF750 is a lineage-specific tumor suppressor in squamous cell carcinoma.
    Oncogene 2017 Apr 20;36(16):2243-2254.
    DOI: 10.1038/onc.2016.377
    PMID: 27819679  [link]

2016

  • Wong RW * and D'Angelo M*
    Linking nucleoporin, mitosis and colon cancer.
    Cell Chem. Biol. 2016 May 19; 23(5):537-9.
    doi: 10.1016/j.chembiol.2016.05.004.
    PMID: 27203373.
  • Klionsky DJ, Abdelmohsen K, Abe A, ... Wong RW et al.
    Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
    Autophagy 2016; 12(1):1-222.
    doi: 10.1080/15548627.2015.1100356.
    PMID: 26799652.

2015

  • Kobayashi A, Hashizume C, Dowaki T and Wong RW *
    Therapeutic potential of mitotic interaction between the nucleoporin Tpr and Aurora kinase A.
    Cell Cycle 2015;14(9):1447-58.
    doi: 10.1080/15384101.2015.1021518.
    PMID: 25789545
  • Wong RW *,Mamede J, and Hope TJ*
    The impact of nucleoporin mediated chromatin localization and nuclear architecture on HIV integration site selection.
    J. Virol. 2015 Oct; 89(19):9702-5.
    doi: 10.1128/JVI.01669-15.
    PMID: 26136574
  • Wong RW *
    Nuclear Pore Complex: From structural view to chemical tools.
    Cell Chem. Biol. 2015 Oct 22; 22(10):1285-1287.
    PMID: 26496682

特許

  • 羽澤 勝治ら，「蛍光プローブ、液相の極性及び粘性を評価する方法、並びに化合物」， 金沢大学， 令和 3年 6月 2日(2021.06.02)　特願2021-093100
  • ウォング リチャード ら，「高速原子間力顕微鏡による細胞小器官の観察のための試料調製方法」， 金沢大学， 平成29年11月20日(2017.11.20)　特願2017-223106 令和元年6月20日(2019.6.20)　特開2019-095243 令和３年１１月2５日(2021.11.25)　特許第6982863号
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